A double-beam rapid-scanning stopped-flow spectrophotometer

Rapid flip-flop of phospholipids in endoplasmic reticulum membranes studied by a stopped-flow approach.

The transbilayer movement of short-chain spin-labeled and fluorescent 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) phospholipid analogs in rat liver microsomes is measured by stopped-flow mixing of labeled microsomes with bovine serum albumin (BSA) solution. Extraction of analogs from the outer leaflet of microsomes to BSA can be directly monitored in conjunction with electron paramagnetic resonance...

Phosphate and ATPase Determination Using the μQuant Scanning Microplate Spectrophotometer

There are a large number of assays in cellular and molecular biology that either produce or consume inorganic phosphate. Therefore, the ability to quantitatively measure these phosphorous compounds can allow the assessment of the associated enzymes. Here we describe the use of the mQuant Scanning microplate spectrophotometer to quantitate ATPase activity by measuring inorganic phosphate in solu...

Optical Filter Transmittance Measurements by a Double-Beam Spectrophotometer for Calibration of Fluorescence Detector AUGER Filter Samples

An experimental setup and procedure for accurate transmittance measurements of candidate filters for the Auger Fluorescence Detector is presented. For this check, we used the Hitachi U-2000 double-beam spectrophotometer (Sph) of the Chemical Department of the University of Athens (UoA). After the calibration of the Sph, we are able to observe transmittance deviations over the area of the filter...

A microcomputer-based high-speed data-acquisition system for a stopped-flow spectrophotometer: PDP-11 architecture interfaced to a commercial instrument

Nonlinear Flow-Induced Flutter Instability of Double CNTs Using Reddy Beam Theory

In this study, nonlocal nonlinear instability and the vibration of a double carbon nanotube (CNT) system have been investigated. The Visco-Pasternak model is used to simulate the elastic medium between nanotubes, on which the effect of the spring, shear and damping of the elastic medium is considered. Both of the CNTs convey a viscose fluid and a uniform longitudinal magnetic field is applied t...